Coding

Part:BBa_K3382001:Design

Designed by: Leung Chung Ki   Group: iGEM20_HK_CPU-WFN-WYY   (2020-10-14)


Metagenome from plant compost


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized to express in E. coli cells and removed restriction sites in the sequence.


Source

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294458/bin/supp_78_5_1556__index.htm

References